44 2033180199

Effect of blood transfusion on TNFα, IL-1, and IL-6 cytokine gene expression in abdominal aortic aneurysm repair patients

Azita Chegini, Samad Valizadeh, Shahram Samiee, Sara Zandpazand, Faranak Behnaz

BACKGROUND: An Abdominal Aortic Aneurysm (AAA or triple A) is a localized enlargement of the aorta in the abdominal region, with a diameter of 3 cm or more and also 50% larger than normal. AAA is the most common form of aortic aneurysm, about 85% of which occur below the kidneys, at the level, or above the kidneys. In case of aortic rupture, the patient's mortality rate will be 85% to 90%. It is estimated that about 0.2% of blood recipients have a systemic inflammatory response. Considering the vital need of aortic aneurysm patients for blood transfusion, we evaluated the gene expression changes of inflammatory cytokines such as interleukin 1 and 6 (IL-1 and 6) and Tumor Necrosis Factor α (TNF-α) in these patients before anesthesia, after anesthesia, at different intervals after transfusion until the patient's discharge time.

MATERIALS AND METHODS: On the time intervals of before anesthesia, after anesthesia, and before blood transfusion in the operating room, 24 hours and 48 hours after blood transfusion (pact cells) in the ICU and surgery department, and at the time of discharge, gene expression sampling was performed. The samples were taken and the total RNA was extracted. To quantitatively measure the genes of transcription factors, Real-time PCR was performed. T-test was used to analyze the gene expression after calculating fold change.

RESULTS: IL-1 gene expression increased in 24 hours after blood transfusion (24 h) (P=0.0376) and discharge time (P=0.0301), after anesthesia (AA) (P=0.235), and 72 hours after blood transfusion (P=0.1667). IL-6 gene expression changes after blood transfusion showed increased expression 24 hours after blood transfusion(P=0.046), and at the time of discharge (P=0.0473). TNF-α gene expression increased after anesthesia (AA) (P=0.699), in 24 hours after blood transfusion (24 h) (P=0.0625), 72 hours after blood transfusion (P=0.0121), and discharge time (P=0.0141).

CONCLUSION: These changes showed IL-1, IL-6, and TNF-α gene expression increased 24 h after transfusion and upon discharge.

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