Malaria is a parasitic disease transmitted by Anopheles mosquitoes. It is considered as a leading cause of mortality among children in sub-Saharan Africa and especially in South Sudan. A valid quantitative estimates of malaria mortality worldwide are useful for monitoring the impact of implementing the prevention and the control of the activities, targeting interventions, and advocacy Most at risk of getting malaria are pregnant women, children under five, HIV positive persons and those who come from areas where the incidence of malaria is very low, for example Nairobi or Khartoum. In couples of years, malaria accounted for 7% of deaths in children aged less than 5 years globally, and 17% of these deaths in sub-Saharan Africa including South Sudan, where it was considered the leading cause of death in Africa.