The prospective study was carried out for detecting the prevalence of HIV in sexually transmitted diseases (S.T.D) cases at Shanti infectious diseases clinic at Vadodara, Gujarat, India from January 2018 to May 2020. Out of 400 cases of sexually transmitted diseases, 317 (79.23 %) were males and 83 cases (20.75%) were females. Screening of HIV test was done by Elisa test and was confirmed by Western blot test.